214854-HRP
GFAP (Glial Fibrillary Acidic Protein) (HRP)
Size
£960.00
- SKU:
- 214854-HRP
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Bovine spinal cord homogenate.
- Clone:
- 1B4
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
- Extra Details:
- Glial fibrillary acidic protein, or GFAP, a class III intermediate filament. During development of the central nervous system, GFAP distinguishes astrocytes from other glial cells. It is involved in various cellular functions, such as cell structure and movement, cell communication and the functioning of the blood-brain barrier. It also plays a role in mitosis by adjusting the filament network present in the cell. Applications: Suitable for use in RIA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: Frozen and paraffin sections. Requires protein digestion pretreatment of paraffin sections e.g. trypsin or pronase. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice