Endothelin B Receptor (MaxLight 650)

Size

£852.00

SKU:: 214810-ML650
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Synthetic peptide corresponding to KANDHGYDNFRSSNN aa424-437 of rat Endothelin B Receptor, conjugated to KLH.
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Extra Details:: MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Specifically Recognizes an epitope within the C-terminal (CT) region of endothelin B receptor (ET-B), a widely distributed transmembrane protein and member of the G-protein coupled receptor 1 family, which acts as a non-specific receptor for endothelin 1, 2, and 3, and mediates its action by G protein association and activation of a phosphatidylinositol-calcium second messenger system. Recognizes epitopes on the cytoplasmic side of the receptor and therefore the cell membrane must be permeable to the antibody. Does not block the binding of endothelins Applications: Suitable for use in Immunofluorescence, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 10-20ug/ml Immunoprecipitation: 20-40ug/ml Immunohistochemistry(Paraffin): 10-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

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