214753
CD86 (B7-2) (PE)
Size
£723.00
- SKU:
- 214753
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- HTLV-1 transformed Lewis-S1 cells.
- Clone:
- 24F
- Format:
- Supplied as a lyophilized powder in PBS, 5% sucrose, 1% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile dH2O. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- CD86, otherwise known as B7-2, is a type I transmembrane protein and member of the Ig superfamily, which acts as a ligand for both CD28 and CD152 (CTLA-4), and is primarily expressed on antigen presenting cells (APCs) including dendritic cells, and also on germinal center B cells and macrophages. Like CD80, CD86 is an accessory molecule which functions in the CD28-CD80/CD86 co-stimulatory pathway, vital for T cell activation, crosstalk between T and B cells, and Th2-mediated Ig production. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: neat, use 10ul to label 1x10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. Hybridoma: P3U1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice