CD8

Size

£691.00

SKU:: 214734
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Clone:: CC63
Format:: Supplied as a liquid in PBS, 0.09% sodium azide
Extra Details:: Reacts with the bovine CD8 antigen expressed by a subset of T lymphocytes. The antibody precipitates molecules of 34kD and 38kD under reducing conditions. CD8 has been reported as being suitable for use on formalin dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques (Gutierrez et al. 1999). Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50. Use 10ul to label 1x10e6 cells in 100ul Immunohistochemistry: Frozen and paraffin sections Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from BALB/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.