CD5 (APC)

Size

£832.00

SKU:: 214710
Application:: Flow Cytometry
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rat
Immunogen:: Concanavilin A activated canine peripheral blood cells.
Clone:: 10B1757 (YKIX322.3)
Format:: Supplied as a lyophilized powder in PBS, 5% sucrose, 1% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile dH2O. Labeled with Allophycocyanin (APC).
Extra Details:: CD5 is a 67kD cell surface type 1 transmembrane glycoprotein also known as Lymphocyte antigen T1, Ly-1 or Leu-1. CD5 is expressed on the surface of T cells and thymocytes, CD5 is also expressed by NK cells at low levels (Huang et al. 2008). CD5, was clustered as canine CD5 in the First Canine Leucocyte Antigen Workshop (Cobbold et al. 1994). In a study of 73 cases of canine chronic lymphocytic leukemia (CLL) CD5 expression was absent on all cases of B cell CLL as defined by CD21 expression and lack of CD3 or other T cell antigen expression (Vernau and Moore 1999). CD5 serves as a useful marker for the discrimination of canine leukemias of differing origins (Villiers 2002). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: Y3/Ag1.2.3 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months at 4°C after receipt. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap. * For APC conjugates: Remove: Dilute required amount only prior to immediate use. >>> if product is to be used neat (i.e., without dilution).

Shipping Conditions:: Blue Ice