CD45 (LCA)

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£576.00

SKU:: 214695
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: COS cells transfected with CD45 gene.
Clone:: UM16-6
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: CD45 is essential for antigen-induced signal transduction through the antigen receptor and as with other PTPase family members, acts in balance with protein tyrosine kinases, causing the dephosphorylation of negative regulatory tyrosine sites. Studies have indicated that dephosphorylation by CD45, is required for the activation of the src-family kinases p56lck and p59fyn. Investigations into the properties of CD45 in chicken models are limited, but there is evidence of the existence of an additional cysteine residue near the transmembrane region. The overall domain structure between mammalian and chicken CD45 appears to be conserved, but the sequence homology between the extracellular regions is very low. Applications: Suitable for use in Flow Cytometry, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution:: Flow Cytometry: 1:100-1:200, 10ul labels 10e6 cells in 100ul Immunohistochemistry: frozen Optimal dilutions to be determined by the researcher. Immunohistochemistry Positive Control Tissue: Chicken lung Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice