214694-HRP

CD45 (HRP)

Size

£947.00

SKU:: 214694-HRP
Application:: IHC-Fr
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Glycoproteins isolated from the T cell line, RL-5.
Clone:: L12/201
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Horseradish peroxidase (HRP).
Extra Details:: Immunoprecipitation was only obtained after cross linking antibody to the labelled cell surface. This gave a band of ~200kD. Applications: Suitable for use in Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Hybridoma: P3.X63.Ag8-U1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.