214694-APC

CD45 (APC)

Size

£947.00

SKU:: 214694-APC
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Glycoproteins isolated from the T cell line, RL-5.
Clone:: L12/201
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Extra Details:: Immunoprecipitation was only obtained after cross linking antibody to the labelled cell surface. This gave a band of ~200kD. Applications: Suitable for use in Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Hybridoma: P3.X63.Ag8-U1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.