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214685-BIOTIN

CD4 (Biotin)

Size

£914.00

SKU:
214685-BIOTIN
Purity:
Purified by Protein G affinity chromatography from tissue culture supernatant
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Rat
Immunogen:
Canine Concanavilin A activated T-cell blasts
Clone:
YKIX302.9
Format:
Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Extra Details:
Clustered at the first Canine Leukocyte Antigen Workshop (CLAW) [Cobbold et al. 1992]. Rat anti canine CD4 has been demonstrated to partially deplete circulating T lymphocytes when administered in vivo, but alone was not sufficient to prolong allograft survival in a canine transplant model (Watson et al. 1993). Uniquely amongst mammals, canine CD4 is expressed by neutrophils as well as by lymphocytes subsets Moore et al. 1992. Rat anti Canine CD4 forms part of a panel of anti canine monoclonal antibodies used extensively in the evaluation of leukemic status in dogs (Villiers 2002). Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Shipping Conditions:
Blue Ice