214684-ML490
CD4 (MaxLight 490)
Size
£856.00
- SKU:
- 214684-ML490
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Bovine thymocytes
- Clone:
- CC30
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
- Extra Details:
- MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. Recognizes a 50kD transmembrane molecule considered to be the bovine homologue of human CD4. The phenotype, tissue distribution and function of T-cells expressing the bovine CD4 antigen are similar to those in other species. However, expression on macrophages has not yet been detected. Applications: Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Immunohistochemistry (Paraffin): Suitable for use on formal dichromate (FD5) fixed paraffin embedded tissues with amplification and antigen retrieval techniques Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice