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214681

CD34 (PE)

Size

£397.00

SKU:
214681
Application:
Flow Cytometry
Purity:
Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:
4°C Do Not Freeze
Supplier:
United States Biological
Host:
Mouse
Immunogen:
Human endothelial cell membrane vesicles.
Clone:
QBEND/10
Format:
Supplied as a lyophilized powder in PBS, 1% BSA, 0.09% sodium azide. Reconstitute with 250ul sterile dH2O. Labeled with R-Phycoerythrin (PE).
Extra Details:
Human CD34 exists as two isoforms, the full length form described here and a truncated isoform lacking the carboxy-terminal of the intracellular domain and containing some alternative sequence in the remaining intracellular region. Binding epitopes on human CD34 have been classified according to their resistance to enzymatic degradation and grouped together using this and competitive binding assays (Lanza et al. 1999). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: NSO myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Lyophilized powder may be stored at 4°C. Reconstitute with sterile dH2O. Aliquot and store at 4°C. Reconstituted product is stable for 6 months as an undiluted liquid. Dilute only prior to immediate use. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Shipping Conditions:
Blue Ice