214666
CD32 (FcRII) (FITC)
Size
£722.00
- SKU:
- 214666
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Bovine FcgRII-transfected COS7 cells
- Clone:
- CCG39
- Format:
- Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
- Extra Details:
- Recognizes the bovine homologue of human CD32, one of a group of Fc receptors belonging to the immunoglobulin superfamily and involved in phagocytosis of opsonized microbes. Bovine CD32 is a single pass type 1 membrane protein of approximately 32kD, expressed on the cell surface of most cells including B-lymphocytes, monocytes, neutrophils and afferent veiled lymph dendritic cells Chattha, K. et al. 2010. It has been shown that expression of bovine CD32 is higher on macrophages than on neutrophils. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat 1:10 Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
- Shipping Conditions:
- Blue Ice