214654-HRP
CD3 (HRP)
Size
£940.00
- SKU:
- 214654-HRP
- Application:
- IP
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Clone:
- MEM-57
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
- Extra Details:
- The TCR complex consists of TCR heterodimers, either a/b or y/b expressed in a mutually exclusive manner, analagous that seen in mice on different T cell lineages. In order for the TCR heterodimer to be expressed on the cell surface it must associate with a minimal CD3 complex. The CD3 complex itself is made up of four subunits, g, d e and z responsible for effecitng signalling upon TCR activation, via a single or multiple, depending on the subunit, immunoreceptor tyrosine-based activation motif (ITAM) present in the CD3 subunit cytoplasmic domain (Kuhns et al. 2006 Dave et al. 1997) Applications: Suitable for use in Immunoprecipitation. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: P3/NS1/1-Ag4-1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice