214654-BIOTIN
CD3 (Biotin)
Size
£940.00
- SKU:
- 214654-BIOTIN
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Clone:
- MEM-57
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
- Extra Details:
- The TCR complex consists of TCR heterodimers, either a/b or y/b expressed in a mutually exclusive manner, analagous that seen in mice on different T cell lineages. In order for the TCR heterodimer to be expressed on the cell surface it must associate with a minimal CD3 complex. The CD3 complex itself is made up of four subunits, g, d e and z responsible for effecitng signalling upon TCR activation, via a single or multiple, depending on the subunit, immunoreceptor tyrosine-based activation motif (ITAM) present in the CD3 subunit cytoplasmic domain (Kuhns et al. 2006 Dave et al. 1997) Applications: Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: P3/NS1/1-Ag4-1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice