214643-FITC

CD28 (FITC)

Size

£965.00

SKU:: 214643-FITC
Purity:: Purified by Ion Exchange chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Chicken Leucocytes
Clone:: AV7
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
Extra Details:: The chicken homologue of mammalian CD28, demonstrating 50% sequence identity and conservation of key functional sequence. In chickens CD28 is expressed by all thymocytes and T cells excluding most of those that are gamma/delta TCR positive (Koskela et al. 1998). The co-stimulatory role of CD28 is important to T cell function in chickens (Arstila et al. 1994). Applications: Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.