214643-BIOTIN

CD28 (Biotin)

Size

£965.00

SKU:: 214643-BIOTIN
Purity:: Purified by Ion Exchange chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Chicken Leucocytes
Clone:: AV7
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Extra Details:: The chicken homologue of mammalian CD28, demonstrating 50% sequence identity and conservation of key functional sequence. In chickens CD28 is expressed by all thymocytes and T cells excluding most of those that are gamma/delta TCR positive (Koskela et al. 1998). The co-stimulatory role of CD28 is important to T cell function in chickens (Arstila et al. 1994). Applications: Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.