CD25 (IL-2R Alpha Chain) (FITC)

Size

£754.00

SKU:: 214617
Application:: Flow Cytometry
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Enriched and stimulated Canine T cells
Clone:: P4A10
Format:: Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Extra Details:: CD25 cell surface, also known at IL-2Ra, a glycoprotein of approximately 55kD expressed primarily by activated T lymphocytes (Abrams et al. 2010). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat 1:10 Optimal dilutions to be determined by the researcher. Hybridoma: P3-653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability:: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.