214616-BIOTIN

CD25 (IL-2R Alpha Chain) (Biotin)

Size

£832.00

SKU:: 214616-BIOTIN
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Enriched and stimulated Canine T cells
Clone:: P4A10
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Extra Details:: CD25 cell surface, also known at IL-2Ra, a glycoprotein of approximately 55kD expressed primarily by activated T lymphocytes (Abrams et al. 2010). Applications: Suitable for use in Flow Cytometry, Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50-1:100 Optimal dilutions to be determined by the researcher. Hybridoma: P3-653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.