CD25 (IL-2R Alpha Chain, Interleukin-2 Receptor Alpha Chain)

Size

£570.00

SKU:: 214615
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Cell membrane preparation of HTLV-1 transformed rabbit T cell line.
Clone:: KEI-alpha1
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: CD5 is a 55kD cell surface antigen, otherwise known as the interleukin-2 (IL-2) receptor alpha chain. In rabbits, CD25 is expressed on activated T lymphocytes. Mouse anti Rabbit CD25 antibody, is reported to inhibit the binding of IL-2 to its receptor (Kotani et al. 1993). Applications: Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:100-1:200. 10ul labels 10e6 cells in 100ul Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Hybridoma: PAI myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.