CD16 (FcRIII)

Size

£550.00

SKU:: 214585
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: A polyclonal population of NK cells
Clone:: KD1
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: CD16 is a 50-65kD cell surface molecule, which is the low affinity receptor for IgG (FcR III). CD16 exists as a transmembranous form (Fc gammaRIIIA, or CD16A) and a glycosyl phosphatidylinositol (GPI) anchored form (Fc gammaRIIIB, or CD16B). CD16A is expressed by NK cells, some T cells, and macrophages (Moretta et al. 1990), whereas CD16B is primarily expressed by granulocytes (Bonecchi et al. 1999). Recognizes both forms of CD16 and will therefore recognize all cell types expressing CD16. Applications: Suitable for use in Flow Cytometry and Functional Assays. Other applications not tested. Recommended Dilutions: Flow Cytometry: 100ul labels 1x10e6 cells in 100ul Fuctional Assays: Removal of sodium azide by dialysis is recommended. Optimal dilutions to be determined by the researcher. Hybridoma: P3U1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.