214502
Apo-SAA1, Recombinant, Human (Serum Amyloid A-1 Protein)
Size
£364.00
- SKU:
- 214502
- Molecular Weight:
- 11.7
- Purity:
- ≥98% (SDS-PAGE, HPLC). Endotoxin: ≤0.1ng/ug (≤1EU/ug)
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a lyophilized powder in 5mM Tris, pH7.6. No preservative added. Reconstitute with sterile ddH2O to 0.1-1mg/ml. For extended storage, the addition of 0.1% BSA is recommended. Do not vortex.
- Extra Details:
- Serum amyloid A proteins (SAA) represents a family of apolipoproteins that circulates in association with high-density lipoproteins (HDL). The level of Apo-SAA, normally 1-5ug/ml in plasma, increases 500-1000 fold within 24 hours of an inflammatory stimulus and, under these conditions, is the most abundant HDL apolipoprotein. The human SAA gene codes for a 122 amino acid nonglycosylated polypeptide, which contains an 18 amino acid N-terminal sequence. Recombinant human Apo-SAA1 is an 11.7kD protein containing 105 amino acid residues. Recombinant protein corresponding to human Apo-Serum Amyloid A1 expressed in E. coli. Swiss/Uniprot: P02735 Molecular Weight: ~11.7kD Amino Acid Sequence: MRSFFSFLGE AFDGARDMWR AYSDMREANY IGSDKYFHAR GNYDAAKRGP GGVWAAEAIS DARENIQRFF GHGAEDSLAD QAANEWGRSG KDPNHFRPAG LPEKY Biological Activity: Determined by its ability to chemoattract human monocytes using a range of 10-100ng/ml. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice