214472-ML650
Aggrecan (AGC1, CSPG1, MSK16, ACAN) (MaxLight 650)
Size
£1,081.00
- SKU:
- 214472-ML650
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified human articular cartilage aggrecan.
- Clone:
- 7D4
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Aggrecan, is a proteoglycan and member of the aggrecan/versican proteoglycan family, which forms a major component of the extracellular matrix (ECM) of both cartilage and the central nervous system (CNS). Studies using a panel of core protein-directed antibodies against human aggrecan, revealed the distribution of different aggrecan isoforms within the CNS, and sub-divided the isoforms into clusters 1-5 accordingly. These studies also showed a difference in the relative abundance of these isoforms when comparing brain and cartilage tissues (Virgintino et al. 2009). Applications: Suitable for use in FLISA, Western Blot, Immunoprecipitation and Immunohistochemistry (frozen/paraffin). Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Positive Control: Cerebral Cortex Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice