214464-ML650
Actin, beta (ACTB and Beta Actin) (MaxLight 650)
Size
£852.00
- SKU:
- 214464-ML650
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to the acetylated N-terminal nonapeptide of beta-cytoplasmic actin, coupled to KLH.
- Clone:
- 4C2
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells. Six different isoforms of actin have been identified in mammals. (Vandekerckhove et al. 1978). Mouse anti Human actin beta, has been used to look at the remodeling of the actin cytoskeleton following sendai virus infection and the specific role of the viral M protein in actin remodelling (Miazza et al. 2011) Applications: Suitable for use in Immunohistochemistry, Western Blot and Immunofluorescence. Other applications not tested. Recommended Dilution: Immunohistochemistry: Frozen Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice