209174

Resistin-Like protein gamma, Recombinant, Mouse, His-Tag (RELM-gamma)

Size

£1,151.00

SKU:: 209174
Molecular Weight:: 11
Purity:: Highly Purified (~95%)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder in 0.05M acetate buffer, pH 4. Reconstitute in 100-200ul of 0.1M acetate buffer, pH 4.
Extra Details:: Resistance to insulin characterizes type 2 diabetes, the most common form of diabetes. There is a strong link between type 2 diabetes and obesity, as most patients tend to be obese. Thiozolidinediones (TZDs), a new class of anti-diabetic, enhances target-tissue sensitivity to insulin. A screen of genes down regulated by TZD in adipocyte led to the discovery of a new protein hormone called resistin (for resistance to insulin). Some other protein related to resistin has been called resistin- related molecules (RELM-alpha and beta). Resistin family of proteins was also identified as proteins (FIZZ1-3, for Found in Inflammatory zone) involved in allegory and inflammation. RELM-alpha/FIZZ1/PMNG1 (Parasite-induced Macrophage Novel Gene 1 (prepeptides: mouse/rat 111aa; ~77% identity) is 63% related to resistin at the C-terminus (38aa). RELM-gamma is a novel member of the resistin-like molecule/found in inflammatory zone (RELM/FIZZ) family in mice and rats. Highest expression in hematopoietic tissues, suggesting a cytokine-like role for RELM-gamma. RELM-gamma-mRNA is detectable in bone marrow, spleen, and lung as well as in peripheral blood granulocytes. RELM-gamma (mouse aa111-117; rat 111aa; Signal peptide aa1-25) is a secreted protein of ~11kD). Source: Recombinant protein corresponding to 86aa from mature mouse RELM-gamma, fused to his-tag at N-terminal, expressed in E. coli. Molecular Weight: ~11kD, due to the tags AA Sequence: TLES IVEKKVKELL ANRDDCPSTV TKTFSCTSIT ASGRLASCPS GMTVTGCACG YGCGSWDIRD GNTCHCQCST MDWATARCCQ LA Applications: Suitable for use in ELISA, Western Blot controls or Standards. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice