209162

Proteinase 3, Human (PRTN3, Neutrophil)

Size

£544.00

SKU:: 209162
Molecular Weight:: 29
Purity:: ≥95%
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 50mM MES, pH 4.5, 700mM sodium chloride.
Extra Details:: Proteinase 3 also known as PRTN3 is an enzyme expressed mainly in neutrophil granulocytes. Its exact role in the function of the neutrophil is unknown but in human neutrophils proteinase 3 contributes to the proteolytic generation of antimicrobial peptides. It is also the epitope of anti-neutrophil cytoplasmic antibodies (ANCAs) of the c-ANCA (cytoplasmic subtype) class, a type of antibody frequently found in the disease Wegener's granulomatosis. Anti-neutrophil cytoplasmic antibodies (ANCAs) are autoantibodies produced in response to neutrophil cytoplasmic enzymes, and evaluating the levels of ANCAs is useful for making a diagnosis of systemic vasculitis such as granulomatosis with polyangiitis (Wegener’s) (GPA) or microscopic polyarteritis (MPA). The majority of p-ANCAs and c-ANCAs have been proven to be equivalent to MPO- and PR3- ANCAs, respectively. Patients with MPA and Churg- Strauss syndrome are positive for p-ANCA, and those with GPA are positive for c-ANCA (3-5). In patients with active systemic GPA, MPA or Churg-Strauss syndrome, ANCAs are reported to be positive in over 80%, 70% or 50% of cases, respectively. Moreover, as ELISA provides a good detection rate and useful results, we analyzed the levels of MPO- and PR3- ANCAs to make a diagnosis of systemic vasculitis in the current clinical setting. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:10K-1:100K Western Blot: 100-500ng/lane Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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