209156

Protein A/G, Recombinant (Agarose)

Size

£576.00

SKU:: 209156
Application:: IP
Purity:: Purified
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Extra Details:: Protein A is a cell wall protein deriving from Staphylococcus aureus which exhibits unique binding properties for IgG from a variety of mammalian species and for some IgM and IgA as well. It binds with the Fc region of immunoglobulins through interaction with the heavy chain. It couples to a wide variety of reporter molecules including fluorescent dyes, enzyme markers, biotin, colloidal gold and radioactive iodine without affecting the antibody binding site. Recombinant Protein A was developed to increase the specificity of the molecule for IgG and is widely used both in research and bioprocessing. Protein G is a bacterial cell wall protein isolated from group G Streptococci. Native Protein G contains two IgG-binding domains and sites for albumin and cell surface binding. The albumin and cell surface binding domains have been eliminated from purified Recombinant Protein G to reduce nonspecific binding and, therefore, can be used to separate IgG from crude samples. Optimal binding occurs at pH 5, although binding is also good at pH 7.0-7.2. Protein G has greater affinity than Protein A for most mammalian IgGs, it may be used for the purification of mammalian IgGs that do not bind well to Protein A. Protein G does not bind to human IgM, IgD and IgA. Source: Recombinant purified Protein-A and Protein G were coupled to Agarose using a unique immobilization chemistry that retains the maximum IgG-binding capacity and minimum Protein-A leaching (~5ng protein/ml). Specificity: Suitable for IP of mouse (IgG1, IgG2a, IgG2b, IgG3, and IgA), rat (IgG1, IgG2a, IgG2b, IgG2c) rabbit and goat polyclonal antibodies and human (IgG1-4). Binding Capacity: ~10-15mg human IgG/ml of Protein A/G-Agarose Applications: Suitable for use in Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunoprecipitation: 20-50ul suspension Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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