209115

Neurotrophin-3, Human, Recombinant (NT-3, Neurotrophic Factor, Nerve Growth Factor-2, NGF-2, HGNF)

Size

£651.00

SKU:: 209115
Molecular Weight:: 13.6
Purity:: Highly Purified (~95%)
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4.
Extra Details:: Neurotrophin-3 is a protein that in humans is encoded by the NTF3 gene. The protein encoded by this gene, NT-3, is a neurotrophic factor in the NGF (Nerve Growth Factor) family of neurotrophins. It is a protein growth factor which has activity on certain neurons of the peripheral and central nervous system; it helps to support the survival and differentiation of existing neurons, and encourages the growth and differentiation of new neurons and synapses. NT-3 was the third neurotrophic factor to be characterized, after nerve growth factor (NGF) and BDNF (Brain Derived Neurotrophic Factor. NT-3 binds three receptors on the surface of cells which are capable of responding to this growth factor: TrkC (pronounced "Track C"), is apparently the "physiologic" receptor, in that it binds with greatest affinity to NT-3. However, NT-3 is capable of binding and signaling through a TrkC-related receptors called TrkB. Finally, NT-3 also binds a second-receptor type besides Trk receptors, called the LNGFR (for "low affinity nerve growth factor receptor). Human and mouse NTF-3 are identical and high degree of sequence conservation on rat, pig, dog, chicken, hamster etc. Source: Recombinant protein corresponding to 240aa from human Neurotrophin-3, expressed in E. coli. Molecular Weight: ~13.6kD, NTF-3 is a homodimer of two 13.6kD monomers of 240aa Biological Activity: The ED50 was measured by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures. It was found to be in the range of 25-50ng/ml. Endotoxin: <0.1ng/ug protein or <1EU/ug protein Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Dry Ice