208708
TWEAK, Recombinant, Human, Animal Free (TNF-related Weak Inducer of Apoptosis, TNFSF12, DR3LG, Apo3-Ligand)
Size
£320.00
- SKU:
- 208708
- Purity:
- ≥98% (SDS-PAGE gel, HPLC). Endotoxin: ≤0.1ng/ug (<1EU/ug)
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a lyophilized powder in 10mM sodium phosphate, pH 7.5, 80mM L-Arginine. Reconsitute with 50ul/250ul sterile ddH2O.
- Extra Details:
- Human TWEAK is a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis. TWEAK, like many members of the TNF family, was able to induce interleukin-8 synthesis in a number of cell lines. Recombinant protein corresponding to human TWEAK, comprising the TNF-like extracellular domain, expressed in E. coli. Biological Activity: Assay #1: The ED50 as determined by the dose- dependent stimulation of IL-8 production by human PBMC is less than 10ng/ml. Assay #2: TWEAK weakly induces the death of HT29 cells when cultured in the presence of IFN-y. The ED50 for this effect is between 30-45ng/ml. Amino Acid Sequence: MKGRKTRARR AIAAHYEVHP RPGQDGAQAG VDGTVSGWEE ARINSSSPLR YNRQIGEFIV TRAGLYYLYC QVHFDEGKAV YLKLDLLVDG VLALRCLEEF SATAASSLGP QLRLCQVSGL LALRPGSSLR IRTLPWAHLK AAPFLTYFGL FQVH Protein Content: Verified by UV Spectroscopy and/or SDS-PAGE gel. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice