208678

Macrophage Colony Stimulating Factor, Recombinant, Human, Animal Free (M-CSF, CSF-1, MGI-IM)

Size

£351.00

SKU:: 208678
Purity:: ~98% (SDS-PAGE gel, HPLC)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder in sodium phosphate, pH 8.0, 75mM sodium chloride. Reconstitute with ddH2O.
Extra Details:: Macrophage Colony Stimulating Factor (M-CSF) is produced by various cell types, including fibroblasts, macrophages and endothelial cells. This colonly stimulating factor is involved in both the development and function of monocytes/macropahges. Human M-CSF is a disulfide-linked homodimer composed of 18.4kD subunits of 158aa residues. Source: Recombinant protein corresponding to human M-CSF, expressed in E. coli. Biological Activity: Determined by its ability to stimulate the proliferation of murine M-NFS-60 cells. The expected ED50 is <1.0ng/ml, corresponding to a specific activity of >1x10e6units/mg. Endotoxin: <0.1ng/ug of protein (<1EU/ug) AA Sequence: MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS Protein Content: Verified by UV Spectroscopy and/or SDS-PAGE gel. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with H2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice