208672

Keratinocyte Growth Factor, Recombinant, Human, Animal Free (KGF, Fibroblast Growth Factor-7, HBGF-7)

Size

£320.00

SKU:: 208672
Purity:: ~95% (SDS-PAGE gel, HPLC)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from PBS. Reconstitute with sterile ddH2O to 0.5-1mg/ml. Do not vortex.
Extra Details:: KGF/FGF-7 is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. KGF/FGF-7 is a mitogen factor specific for epithelial cells and keratinocytes. KGF/FGF-7 signals through FGFR 2b. KGF/FGF-7 plays a role in kidney and lung development, angiogenesis, and wound healing. Recombinant human KGF/FGF-7 is a 18.9kD protein consisting of 163 amino acid residues. Source: Recombinant protein corresponding to human KGF expressed in E. coli. Biological Activity: Determined by the dose-dependent stimulation of thymidine uptake by KGF-responsive BaF3 cells. The expected ED50 is <10ng/ml, corresponding to a specific activity of >1x10e5units/mg. AA Sequence: CNDMTPEQMA TNVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNNYNIMEIR TVAVGIVAIK GVESEFYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHNGG EMFVALNQKG IPVRGKKTKK EQKTAHFLPM AIT Endotoxin: <0.1ng/ug of protein (<1EU/ug) Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice