208661

IL-6, Recombinant, Human, Animal Free (26kD protein, IFN-b2, B Cell Differentiation Factor, BCDF, BSF-2, HPGF, HSF, MGI-2)

Size

£320.00

SKU:: 208661
Molecular Weight:: 26
Purity:: ~98% (SDS-PAGE gel, HPLC)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder in 10mM Sodium Phosphate, pH 7.5. Reconstitute with ddH2O.
Extra Details:: The IL-6 receptor system consists of two chains, IL-6R alpha and gp130. The IL-6R alpha is the binding component speciﬁc to the IL-6, but does not transduce a signal alone. On the contrary, gp130 transmits signals not only of IL-6, but also IL-6 related cytokines, i.e. LIF, OSM, CNTF, IL-11 and CT-1. The low afﬁnity binding sites for IL-6, is composed of IL-6R alpha alone. At low concentrations, soluble IL-6R alpha acts as an agonist of IL-6 activity. Recombinant human sIL-6R alpha is a 37.6kD protein, consisting of the extra cellular domain of the sIL-6R alpha chain (338 amino acids). Source: Recombinant protein corresponding to human IL-6 expressed in E. coli. Molecular Weight: ~26kD Biological Activity: Determined by its ability to stimulate the proliferation of the IL-6 dependent mouse 7TD1 cells. The expected ED50 is <0.1ng/ml, corresponding to a specific activity of >1x10e7units/mg. AA Sequence: PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM Protein Content: Verified by UV Spectroscopy and/or SDS-PAGE gel. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice