208611

EGF, Recombinant, Mouse, Animal Free (Epidermal Growth Factor, Urogastrone, URG)

Size

£345.00

SKU:: 208611
Purity:: ≥98% (SDS-PAGE gel, HPLC). Endotoxin: ≤0.1ng/ug (<1EU/ug)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder in PBS, pH 8.5. Reconstitute with ddH2O.
Extra Details:: Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Recombinant murine EGF is a 6.0kD protein containing 53 amino acid residues. Source: Recombinant protein corresponding to Mouse EGF expressed in E. coli. Biological Activity: Determined by its ability to stimulate the proliferation of mouse Balb/c 3T3 cells. The expected ED50 is <0.1ng/ml, corresponding to a specific activity of >1x10e7units/mg. AA Sequence: NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR Protein Content: Verified by UV Spectroscopy and/or SDS-PAGE gel. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with H2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.