208527

Fas Ligand, Recombinant, Human (FasL, APO-1L, CD95L, CD178, TNFSF6) (Multimeric)

Size

£544.00

SKU:: 208527
Molecular Weight:: 40
Purity:: ≥95% (SDS-PAGE). Endotoxin: <0.02EU/ug (LAL test, Lonza)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder in PBS. Reconstitute with 100ul sterile ddH2O.
Extra Details:: Multimeric Fas Ligand is a high activity construct in which two trimeric FasL are artificially linked via the collagen domain of ACRP30. This construct very effectively mimics the natural membrane-assisted aggregation of FasL in vivo. FasL is a cytokine that binds to TNFRSF6/Fas, a receptor that transduces the apoptotic signal into cells. It is involved in cytotoxic T cell mediated apoptosis and in T cell development. Binds to human and mouse Fas. Recombinant protein corresponding to aa139-281 from human FasL, fused at N-terminus to mouse ACRP30headless (aa18-111) and a FLAG-tag., expressed in HEK 293 cells. Molecular Weight: ~40kD Biological Activity: Induces apoptosis of human Jurkat T cells at a concentration of <0.2ng/ml. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.