207791
Mdc1 (Mediator of DNA Damage Checkpoint Protein 1, 6820401C03, AA413496, Nfbd1, mKIAA0170) (PE)
Size
£800.00
- SKU:
- 207791
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant GST fusion protein corresponding to mouse Mdc1 at C-terminal.
- Clone:
- P2B11
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 50% glycerol. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1 By similarity. Applications: Suitable for use in Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
- Shipping Conditions:
- Blue Ice