207439

VEGF 164, Recombinant, Mouse, 164aa (Vegfa, Vascular Endothelial Growth Factor A, VEGF-A, Vascular Permeability Factor, VPF, Vegf)

Size

£460.00

SKU:: 207439
Molecular Weight:: 48
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder. Reconstitute with 50mM acetic acid.
Extra Details:: Mouse vascular endothelial growth factor 164 (VEGF 164) is produced as a homodimer. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. It is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. In addition to its action as a mitogen it is a potent vascular permeability factor (VPF) in vivo and is also a chemoattractant for monocytes and endothelial cells. It induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It may play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis. Source: Recombinant protein corresponding to 164aa from mouse VEGF 164, expressed in Sf9 cells. Molecular Weight: ~48kD Biological Activity: The ED50 for stimulation of cell proliferation by human umbilical vein endothelial cells for VEGF 164 has been determined to be in the range of 1-5ng/ml. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with 50mM acetic acid. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice