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207355

PROX1 (Prox-1, Prospero Homeobox Protein 1)

Size

£576.00

SKU:
207355
Purity:
Purified by Protein A affinity chromatography.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Rabbit
Immunogen:
Recombinant protein corresponding to aa547-737 from human PROX1.
Format:
Supplied as a lyophilized powder. Reconstitute with sterile dH2O to 0.1-1mg/ml.
Extra Details:
Expression of PROX1 in blood endothelial cells induces expression of other lymphatic marker genes. Together with podoplanin, PROX1 can be used to reliably distinguish lympathic vessels from blood vessels. PROX1 is expressed in CNS, eye, pancreas, liver and heart and it is one of the most specific and reliable markers for lymphatic endothelial cells. The highly conserved C-terminal part of the homeobox transcription factor PROX1 was produced in E. coli. It was not tested for activity and can be used as positive control e.g. in Western analysis. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-5ug/ml Immunohistochemistry (frozen sections): 1:750-1000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Conditions:
Blue Ice