207346
Podoplanin (Glycoprotein 36, PA2.26 Antigen, Aggrus)
Size
£735.00
- SKU:
- 207346
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- MDCK cells stably expressing Podoplanin.
- Clone:
- 18H5
- Format:
- Supplied as a lyophilized powder. Reconstitute with sterile dH2O to 0.1-1mg/ml.
- Extra Details:
- Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma and brain tumors. Podoplanin is the ligand for C-type lectin-like receptor 2 (CLEC-2). Their association is dependent on sialic acid on O-glycans of podoplanin. Through its association with CLEC-2, podoplanin induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth. Applications: Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1-10ug/ml Western Blot: 1-5ug/ml Immunohistochemistry: 6-30ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice