207331

PARP3, Recombinant, Human, His-Tag (Poly [ADP-ribose] Polymerase 3, PARP-3, hPARP-3, IRT1, NAD(+) ADP-ribosyltransferase 3, ADPRT-3, Poly[ADP-ribose] Synthase 3, pADPRT-3, ADPRT3, ADPRTL3)

Size

£492.00

SKU:: 207331
Purity:: ≥98% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 50mM TRIS-HCl, pH 7.5, 100mM sodium chloride, 50mM imidazole, 0.2% NP-40, 10% glycerol.
Extra Details:: PARP-3 is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. May link the DNA damage surveillance network to the mitotic fidelity checkpoint. Negatively influences the G1/S cell cycle progression without interfering with centrosome duplication. May be involved in the regulation of PRC2 and PRC3 complex-dependent gene silencing. Full length recombinant protein corresponding to human PARP-3, fused to a HA-tag and a His-tag, expressed in Sf21 cells. Biological Activity: ≥10U/mg (only monoADP-ribosyl transferase activity) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.