207329

PARP1, Recombinant, Human, His-Tag, Myc-Tag (ARTD1, Poly(ADP-ribose) Polymerase 1, ADP-ribosyltransferase Diphtheria Toxin-like 1, NAD(+) ADP-ribosyltransferase 1)

Size

£492.00

SKU:: 207329
Purity:: ≥98% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 50mM TRIS-HCl, pH 7.5, 100mM sodium chloride, 50mM imidazole, 0.2% NP-40, 10% glycerol.
Extra Details:: PARP-1 (ARTD1) is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PARP-1 positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. It forms a complex with EEF1A1 and TXK that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Full length recombinant protein corresponding to aa1-1014 from human PARP-1, fused to MYC and His-tag, expressed in Sf21 cells. Biological Activity: ≥1200U/mg protein Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.