207328
PARP1, E998K Mutant, Recombinant, Human, His-Tag (Poly [ADP-ribose] Polymerase 1, PARP-1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] Synthase 1, ADPRT, PPOL)
Size
£472.00
- SKU:
- 207328
- Purity:
- ≥95% (SDS-PAGE)
- Storage Conditions:
- -70°C
- Supplier:
- United States Biological
- Format:
- Supplied as a liquid in 50mM TRIS-HCl, pH 7.5, 100mM sodium chloride, 50mM imidazole, 0.2% NP-40, 10% glycerol.
- Extra Details:
- PARP-1 (ARTD1) is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PARP-1 positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. It forms a complex with EEF1A1 and TXK that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. PARP-1 (E998K mutant) is an inactive form of PARP-1 which can be used as a control compound. Recombinant protein corresponding to full-length inactive mutant E998K of human PARP-1, fused to HA-tag and His-tag, expressed in Sf21 cells. Biological Activity: 0.5% of wild type PARP-1 Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Dry Ice