207322

PARG, Recombinant, Human, His-Tag (Poly(ADP-ribose) Glycohydrolase)

Size

£636.00

SKU:: 207322
Purity:: ~95% (SDS-PAGE)
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 100mM sodium chloride, 0.2% NP-40, 50mM imidazole, 10% glycerol
Extra Details:: Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase. PARG hydrolyzes poly(ADP-ribose) at glycosidic (1''-2') linkage of ribose-ribose bond to produce free ADP-ribose. Recombinant protein corresponding to human PARG, fused to His-tag, expressed in Sf21 cells. Swiss/UniProt Accession: Q86W56. Biological Activity: ~100ng is required for poly(ADP-ribose) degradation assays Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.