207287
NAD Nucleotidase, Recombinant, Haemophilus influenzae (NadN, NAD Pyrophosphatase/ 5'-nucleotidase)
Size
£619.00
- SKU:
- 207287
- Molecular Weight:
- 63
- Purity:
- ~98% (SDS-PAGE)
- Storage Conditions:
- -70°C
- Supplier:
- United States Biological
- Format:
- Supplied as a liquid in 5mM KH2PO4, pH 6.8, 100mM TRIS-HCl, pH 8.5, 10mM magnesium chloride.
- Extra Details:
- NadN (NAD nucleotidase) is a periplasmic enzyme from Haemophilus influenzae, a major pathogen of the respiratory tract in humans that has developed the capability to exploit host NAD(P) for its nicotinamide dinucleotide requirement. NadN plays a central role by degrading NAD into adenosine and NR (nicotinamide riboside), NMN to nicotinamide riboside and AMP to adenosine. It shows a broad substrate specificity, recognizing either mono- or di-nucleotide nicotinamides and different adenosine phosphates with a maximal activity on 5'-adenosine monophosphate. It can be used as an economically alternative to produce NR (nicotinamide riboside). Catalytic activity: NAD=AMP + NMNe+NR+PPi AMPe+Pi NMN=NR+Pi. Source: Recombinant protein corresponding to full-length Haemophilus influenzae NAD Nucleotidase, without the signal peptide corresponding to the first 26aa, expressed in E. coli. Molecular Weight: ~63kD Biological Activity: With AMP: 856.4nmol of Ade/min/mg of protein With NMN: 110,3nmol of NR/min/mg of protein With NAD: 5.4nmol of Ade/min per mg of protein | 5.3 nmol of NR/min/mg of protein Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Dry Ice