207286
NAD Kinase, Recombinant, Mycobacterium tuberculosis, His-Tag (Probable Inorganic Polyphosphate/ATP-NAD kinase, Poly(P)/ATP NAD Kinase 1, ppnK1, EC 2.7.1.23)
Size
£544.00
- SKU:
- 207286
- Molecular Weight:
- 33
- Purity:
- ~98% (SDS-PAGE)
- Storage Conditions:
- -70°C
- Supplier:
- United States Biological
- Format:
- Supplied as a liquid in 10mM TRIS, pH 8.0, 1mM MgCl2, 0.5mM EDTA, 1mM DTT, 1mM PMSF.
- Extra Details:
- NAD kinase catalyzes the transfer of a phosphate group from ATP to NAD+ to generate NADP+, which in its reduced form acts as an electron donor for biosynthetic reactions. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. NAD kinase from Mycobacterium tuberculosis is not ATP-dependent but has a broader substrate specificity than human NAD kinase. It can economically produce NADP+ by using other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus. Was identified as a high-confidence drug target. Catalytic activity: ATP + NAD+ = ADP + NADP+ Source: Recombinant protein corresponding to Mycobacterium tuberculosis NAD kinase, fused to His-tag at N-terminal, expressed in E. coli. Molecular Weight: ~33kD Biological Activity: ~1.2 U/mg protein. (Substrate: NAD (+ ATP)) ~3.8 U/mg protein. (Substrate: NAD (+ poly(P)) One unit is defined as the amount of enzyme that synthesizes 1umol nicotinamide adenine dinucleotide phosphate (NADP) per min. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Dry Ice