207264
Lymphotoxin beta Receptor (LTBR, LT-beta-R, Tumor Necrosis Factor C Receptor, Tumor Necrosis Factor Receptor Superfamily Member 3, TNF-RIII, D12S370, TNFCR, TNFR3, TNFRSF3) (Preservative Free)
Size
£552.00
- SKU:
- 207264
- Application:
- Flow Cytometry
- Purity:
- ≥95% (SDS-PAGE). Purified from concentrated hybridoma tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to mouse LTbeta R, cysteine-rich region of aa31-221.
- Clone:
- 4H8 WH2
- Format:
- Supplied as a liquid in PBS, 10% glycerol. No preservative added.
- Extra Details:
- The LTbeta R activates two different NF-kappaB pathways that lead to distinct patterns of gene induction, including selected chemokines and the cytokine BAFF, which is essential for the survival of mature B lymphocytes. LTbeta R activates the classical NF-kappaB (relA/p50) pathway, like the type 1 TNF receptor (TNFR1), that regulates proinflammatory genes, like the chemokine MIP1beta. However, LTbeta R, unlike TNFR1, also activates the processing of p100 to form RelB/p52 complexes, which activate genes involved in lymphoid organ formation and lymphocyte survival. LTbeta R is an agonist that can be used for the investigation of the regulation of BAFF (BlyS), chemokines and integrins using in vivo and tissue culture models, the development of NK cells and NK T cells, to study the regulation of NF-kappaB family of transcription factors in regulation of inflammation and homeostasis, particularly RelB NF- kappaB2 pathway. For use as an agonist the MAb to LTbeta R is added to cell cultures at 2ug/ml. For in vivo use, mice are injected intraperitoneally with 50ug of agonistic MAb to LTbeta R in sterile phosphate saline buffer. Applications: Suitable for use in Flow Cytometry and Functional Applications. Other applications not tested. Recommended Dilution: Functional Applications: Agonist inducing BAFF, chemokines and integrins in vitro and in vivo Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice