207222

HGF, Recombinant, Human, 692aa (Hepatocyte Growth Factor, Hepatopoietin-A, Scatter Factor, SF, HPTA)

Size

£338.00

SKU:: 207222
Molecular Weight:: 78
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder. Reconstitute with sterile dH2O to 0.1mg/ml.
Extra Details:: Human hepatocyte growth factor (HGF) is synthesized as a biologically inactive single chain precursor, which is cleaved by a specific, extracellular serum serine protease to a fully active heterodimer. It stimulates the motility and invasion of several cancer cell types and can induce angiogenesis. All biological responses induced by HGF are elicited by binding to its transmembrane tyrosine kinase receptor. HGF function is essential for normal development. Knockout studies have demonstrated that both ligand and receptor deficient mice display an embryonic lethal phenotype. HGF synergizes with basic FGF in the induction of angiogenesis. HGF is a potent mitogen for mature parenchymal hepatocyte cells and is a pleiotrophic cytokine. It acts as growth factor for a broad spectrum of tissues and cell types. It has no detectable protease activity. It is an activating ligand for the receptor tyrosine kinase MET by binding and promoting its dimerization. Recombinant protein corresponding to 692aa from human HGF, expressed in Sf9 cells. Molecular Weight: ~78kD Biological Activity: The activity was assayed for scattering activity in the MDCK cell assay. The ED50 for this effect is typically at 1-5ng/ml. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice