207207

GCN5, Recombinant, Human, His-Tag (GCN5L2, Histone Acetyltransferase GCN5, General Control of Amino Acid Synthesis Protein 5-like 2, Histone Acetyltransferase KAT2A, HsGCN5, Lysine Acetyltransferase 2A, STAF97, HGCN5)

Size

£452.00

SKU:: 207207
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 50mM TRIS-HCl, pH 7.5, 100mM sodium chloride, 0.2% NP-40, 50mM imidazole, 10% glycerol.
Extra Details:: GCN5 functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Acetylation of histones gives a specific tag for epigenetic transcription activation. It has significant histone acetyltransferase activity with core histones, but not with nucleosome core particles. In case of HIV-1 infection, it is recruited by the viral protein Tat. It regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. GCN5 is a component of the Ada Two-A containing (ATAC) complex, a complex with histone acetyltransferase activity on histones H3 and H4. Source: Recombinant protein corresponding to human GCN5, fused to a His-tag, expressed in Sf21 cells. Biological Activity: ~200-300ng are required for standard HAT assay Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.