207182
Flagellin, NLRC4 Mutant, Recombinant, aa1-495, His-Tag (Phase 1-I Flagellin, fliC, flaF, hag, STM1959)
Size
£384.00
- SKU:
- 207182
- Purity:
- ≥95% (SDS-PAGE). Endotoxin: <0.01EU/ug (LAL)
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a lyophilized powder in PBS. Reconstitute with 100ul sterile dH2O.
- Extra Details:
- Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) or the intracellular NLRC4 protein. The Flagellin (NLRC4 Mutant)(rec.) is only detected by TLR5 not by NLRC4. Recombinant protein corresponding to the native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028, aa1-495, contains a small deletion at the C-terminus of the protein required for NLRC4 activation, fused to His-tag at N-terminal, expressed in E. coli. Biological Activity: Activation of TLR5 in human epithelial cell assays based on NF-kB luciferase fusions, in vivo on expression of CCL20 or IL-6. TLR5 can be stimulated in vitro by Flagellin (NLRC4 mutant) at a concentration range of 5-50ng/ml and in vivo at a starting concentration of 0.1-1ug/mouse. Does not activate NLRC4 as tested in bone-marrow mouse macrophages. It can be used as negative control for inflammasome signaling. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice