207135

Crebbp, Recombinant, Mouse, Highly Active, His-Tag (CREB-binding Protein, Cbp)

Size

£468.00

SKU:: 207135
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 50mM TRIS-HCl, pH 7.5, 100mM sodium chloride, 0.2% NP-40, 50-100mM imidazole, 10% glycerol.
Extra Details:: CREB-binding protein acetylates histones, giving a specific tag for transcriptional activation. It also acetylates non-histone proteins, like NCOA3 and FOXO1. It binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1 in the presence of EP300. Source: Full length, recombinant protein corresponding to mouse CREB-binding protein, fused to His-tag at C-terminal, expressed in Sf21 cells. Biological Activity: ~50-100ng are required for standard HAT assay. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.