207109
CD275, Recombinant, Human, aa1-256 (B7-H2, ICOS Ligand, B7-like Protein Gl50, B7RP-1)
Size
£576.00
- SKU:
- 207109
- Purity:
- ~98% (SDS-PAGE)
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a lyophilized powder in PBS. Reconstitute with sterile PBS to 100ug/ml.
- Extra Details:
- Human B7-H2 is a member of the growing B7 family of immune costimulatory proteins. Other family members include B7-1, B7-2, B7-H1 (PD-L1), PD-L2 and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, the extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. B7-H2 has been identified as the ligand for ICOS, a member of the CD28 family of costimulatory receptors. Human B7-H2 is expressed constitutively on resting B cells, dendritic cells and at low levels on monocytes. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation. Source: Recombinant protein corresponding to aa1-256 from human CD275, fused to the N-terminus of the Fc region of mouse IgG2a, expressed in CHO cells. Biological Activity: Measured by its ability to stimulate human T cell proliferation in the presence of anti-CD3. Endotoxin: ~0.06EU/ug protein (LAL test; Lonza) Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice