Asc (Pycard, Target of Methylation-induced Silencing 1, TMS1, Caspase Recruitment Domain-containing Protein 5, Apoptosis-associated Speck-like Protein Containing CARD, CARD5)

Size

£607.00

SKU:: 207063
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to human Asc at N-terminal.
Format:: Supplied as a liquid in PBS. No preservative added.
Extra Details:: Asc promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. It is a component of the inflammasome, a protein complex which also includes NLRP3/NALP3, CARD8 and CASP1 and whose function is the activation of proinflammatory caspases. 207063 can be used to measure inflammasome activation in vivo, detecting Asc speck formation by immunofluorescence and by Flow Cytometry. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry (paraffin sections): 1:500 Immunocytochemistry: 1:200 Immunoprecipitation: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.